Microscope OpenCV and NumPy nodes

Pack ID: microscope_opencv · version 1.0.0.

Declared runtime dependencies:

numpy>=2,<3
opencv-python-headless>=4.12,<5

Grayscale

Stable type ID: microscope_opencv.grayscale
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`image`	`image`	Yes

Outputs

Port	Type
`gray`	`gray`

Parameters

Name	Type	Default	Constraints / notes
—	None	—	—

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Color-space channels

Stable type ID: microscope_opencv.color_channels
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`image`	`image`	Yes

Outputs

Port	Type
`first`	`gray`
`second`	`gray`
`third`	`gray`

Parameters

Name	Type	Default	Constraints / notes
`space`	`enum`	`HSV`	label: Color space; values: `["HSV", "Lab"]`

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Gaussian blur

Stable type ID: microscope_opencv.gaussian
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`gray`	`gray`	Yes

Outputs

Port	Type
`gray`	`gray`

Parameters

Name	Type	Default	Constraints / notes
`sigma`	`number`	`1`	label: Sigma (pixels); min: `0.1`; max: `20`

Execution requirements: {"mode": "region", "version": 1}

Threshold

Stable type ID: microscope_opencv.threshold
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`gray`	`gray`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
`threshold`	`number`	`128`	label: Threshold; min: `0`; max: `255`
`invert`	`boolean`	`True`	label: Select darker pixels
`otsu`	`boolean`	`False`	label: Use Otsu threshold

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Adaptive threshold

Stable type ID: microscope_opencv.adaptive_threshold
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`gray`	`gray`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
`window`	`integer`	`31`	label: Odd window size; min: `3`; max: `255`; step: `2`
`offset`	`number`	`5`	label: Offset; min: `-100`; max: `100`

Execution requirements: {"mode": "region", "version": 1}

Morphology

Stable type ID: microscope_opencv.morphology
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`mask`	`mask`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
`operation`	`enum`	`opening`	label: Operation; values: `["opening", "closing", "erosion", "dilation"]`
`radius`	`integer`	`2`	label: Radius (pixels); min: `0`; max: `64`
`iterations`	`integer`	`1`	label: Iterations; min: `1`; max: `10`

Execution requirements: {"mode": "region", "version": 1}

Connected components

Stable type ID: microscope_opencv.connected_components
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`mask`	`mask`	Yes

Outputs

Port	Type
`labels`	`labels`
`measurements`	`table`

Parameters

Name	Type	Default	Constraints / notes
`connectivity`	`enum`	`8`	label: Connectivity; values: `["4", "8"]`
`max_measurements`	`integer`	`1000`	label: Maximum measurement rows; min: `0`; max: `5000`

Execution requirements: {"mode": "region", "version": 1}

Filter components

Stable type ID: microscope_opencv.filter_components
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`mask`	`mask`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
`min_area`	`integer`	`50`	label: Minimum area (pixels); min: `0`; max: `4194304`
`max_area`	`integer`	`4194304`	label: Maximum area (pixels); min: `1`; max: `4194304`

Execution requirements: {"mode": "region", "version": 1}

Invert mask

Stable type ID: microscope_opencv.invert_mask
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`mask`	`mask`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
—	None	—	—

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Combine masks

Stable type ID: microscope_opencv.mask_arithmetic
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`a`	`mask`	Yes
`b`	`mask`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
`operation`	`enum`	`intersection`	label: Operation; values: `["intersection", "union", "difference", "xor"]`

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Blend images

Stable type ID: microscope_opencv.image_blend
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`a`	`image`	Yes
`b`	`image`	Yes

Outputs

Port	Type
`image`	`image`

Parameters

Name	Type	Default	Constraints / notes
`weight`	`number`	`0.5`	label: First image weight; min: `0`; max: `1`

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Tissue statistics

Stable type ID: microscope_opencv.tissue_statistics
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

No description is recorded in this node schema.

Inputs

Port	Type	Required
`mask`	`mask`	Yes

Outputs

Port	Type
`measurements`	`table`
`fraction`	`number`

Parameters

Name	Type	Default	Constraints / notes
—	None	—	—

Execution requirements: {"mode": "region", "version": 1}

Mask to ROI polygons

Stable type ID: microscope_opencv.mask_to_roi
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

Extract bounded contours, preserving disconnected components and holes in WSI level-0 coordinates.

Inputs

Port	Type	Required
`mask`	`mask`	Yes

Outputs

Port	Type
`rois`	`roi`

Parameters

Name	Type	Default	Constraints / notes
`simplify`	`number`	`0.75`	label: Simplify (source px); min: `0`; max: `8`

Execution requirements: {"mode": "region", "version": 1}

ROI polygons to mask

Stable type ID: microscope_opencv.roi_to_mask
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

Rasterize WSI-coordinate polygons on the current spatial grid, including polygon holes.

Inputs

Port	Type	Required
`image`	`image`	Yes
`rois`	`roi`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
—	None	—	—

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "mask"}

Mask threshold

Stable type ID: microscope_opencv.probability_threshold
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

Convert a probability raster to a binary mask on the identical spatial grid.

Inputs

Port	Type	Required
`probability`	`probability`	Yes

Outputs

Port	Type
`mask`	`mask`

Parameters

Name	Type	Default	Constraints / notes
`threshold`	`number`	`0.5`	label: Threshold; min: `0`; max: `1`

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "mask"}

Apply ROI

Stable type ID: microscope_opencv.apply_roi
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

Fill image pixels outside WSI-coordinate ROI polygons with white on the current grid.

Inputs

Port	Type	Required
`image`	`image`	Yes
`rois`	`roi`	Yes

Outputs

Port	Type
`image`	`image`

Parameters

Name	Type	Default	Constraints / notes
—	None	—	—

Execution requirements: {"mode": "tile_local", "version": 1, "tile_size": 1024, "target_mpp": null, "context_padding": 0, "overlap": 0, "merge": "core"}

Combine ROIs

Stable type ID: microscope_opencv.roi_boolean
Version: 1
Required pack: Microscope OpenCV and NumPy nodes (microscope_opencv 1.0.0)

Combine polygons on the connected image grid; output coordinates remain WSI level-0 pixels.

Inputs

Port	Type	Required
`image`	`image`	Yes
`a`	`roi`	Yes
`b`	`roi`	Yes

Outputs

Port	Type
`rois`	`roi`

Parameters

Name	Type	Default	Constraints / notes
`operation`	`enum`	`intersection`	label: Operation; values: `["intersection", "union", "difference"]`

Execution requirements: {"mode": "region", "version": 1}

Microscope OpenCV and NumPy nodes ​

Grayscale ​

Color-space channels ​

Gaussian blur ​

Threshold ​

Adaptive threshold ​

Morphology ​

Connected components ​

Filter components ​

Invert mask ​

Combine masks ​

Blend images ​

Tissue statistics ​

Mask to ROI polygons ​

ROI polygons to mask ​

Mask threshold ​

Apply ROI ​

Combine ROIs ​

Microscope OpenCV and NumPy nodes

Grayscale

Color-space channels

Gaussian blur

Threshold

Adaptive threshold

Morphology

Connected components

Filter components

Invert mask

Combine masks

Blend images

Tissue statistics

Mask to ROI polygons

ROI polygons to mask

Mask threshold

Apply ROI

Combine ROIs